BeNa Culture Collection
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| Culture medium | BNCC352246 |
| Description | R2A Agar |
| Composition | Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K2HPO4:0.3g, MgSO4:0.024g, Sodium Pyruvate(C3H3NaO3):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2 mm, circular in shape with regular edges and opaque. The obverse side is creamy white and pale in color, centrally raised with a smooth, glossy surface and moist texture, easily picked off. No pigment production is observed. Gram-negative (red), rod-shaped bacteria, purity: pure. |
| Sharing mode | Public welfare sharing |
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