Pseudomonas aeruginosa|134064 |BNCC

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Pseudomonas migulae

Culture medium	BNCC352246
Description	R2A Agar
Composition	Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K₂HPO₄:0.3g, MgSO₄:0.024g, Sodium Pyruvate(C₃H₃NaO₃):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2 mm, circular in shape with regular edges and opaque. The obverse side is creamy white and pale in color, centrally raised with a smooth, glossy surface and moist texture, easily picked off. No pigment production is observed. Gram-negative (red), rod-shaped bacteria, purity: pure.
Sharing mode	Public welfare sharing