Atypical Vibrio parahaemolyticus|379565 |BNCC

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Veillonella atypica

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Prepare 1-2 fresh plates mentioned above (place them in an anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving, transfer it back into the liquid test tube and mix well; ④ Take 0.2mL of bacterial suspension and pour it into a Agar plate. Apply evenly and do not draw lines. Repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, G - (red), cocci, purity: pure
Sharing mode	Public welfare sharing