Mysterious subspecies of Bacillus subtilis|139274 |BNCC

Essential Information Related Products

Clavibacter michiganensis subsp. insidiosus

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	26 ℃; 3-4 days; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, yellow on the front, light in color, raised in the middle, gray on the surface, easy to pick up, G (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing