BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃; 2-3 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | A small filamentous fungus. On synthetic PDA medium, the colony is white and cotton‑like, spreading toward the edge of the Agar plate. In later stages, the colony turns gray, while the reverse side of the medium shows a pale yellow coloration. |
| Sharing mode | Public welfare sharing |
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