Oriental anaerobic bacteria|152987 |BNCC

Essential Information Certificate Related Products

Amycolatopsis orientalis

Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Disinfect the surface of the carrier tablet and open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a bus straw, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, rough on the surface, dry texture, G+(blue purple), Bacillus subtilis
Sharing mode	Public welfare sharing

Pseudomonas orientalis

Storage conditions : 2~8 ℃

No. : 152987

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, ISP-1,3-5 days, ISP-1: tryptone 5.0g, yeast extract 3.0g, agar 20.0g, distilled water 1.0L,pH 7.0-7.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 3-5days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 3-5days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Untidy Transparency: Opaque Color: White Protrusion: Middle Raised Surface: Rough Texture: Dry
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate