BeNa Culture Collection
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| Culture medium | BNCC352247 |
| Description | Gaoshi Synthesis 1 Agar |
| Composition | Soluble Starch:20.0g, NaCl:0.5g, FeSO4:0.01g, KNO3:1.0g, K2HPO4:0.5g, MgSO4:0.5g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30 ℃; 48-72 hours; aerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony has a diameter of 1-2mm, is circular, with irregular edges, opaque, golden yellow on the front, raised in the middle, rough and dark on the surface, hard texture, dry texture, G+(blue purple), curved rod-shaped, purity: pure |
| Sharing mode | Public welfare sharing |
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