Bacillus subtilis var. niger NRS 1221A|260902 |BNCC

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Bacillus atrophaeus Nakamura

Culture medium	BNCC362137
Description	Tyrosine Agar Medium
Composition	Tryptone:0.5g, Glucose:0.5g, NaCl:0.5g, K₂HPO₄:0.5g, MgSO₄.7H₂O:0.5g, L-Asparagine:0.5g, L-Tyrosine:0.5g, Dry Yeast:0.5g, FeSO₄.7H₂O:0.00136g, CuCl₂.2H₂O:0.000027g, CoCl₂.6H₂O:0.00004g, Na₂MoO₄.2H₂O:0.000025g, ZnCl₂:0.00002g, H₃BO₃:0.00285g, MnCl₂.4H₂O:0.0018g, Sodium Tartrate(C₄H₄Na₂O₆):0.00177g, Agar:20g, pH:7.0±0.2(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, darker color, flat shape, rough surface, wrinkled surface, dry texture, purity: pure, G+(blue purple), Bacillus subtilis
Sharing mode	Public welfare sharing