Aspergillus oryzae|185854 |BNCC

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Aspergillus oryzae

Subculture procedure	① Prepare 2 plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, produce a large number of spores, produce yellow spores, irregular edges, opaque, golden yellow front, flat shape, rough surface, wrinkled surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 185854

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, Chapman's agar, 5-7 days. Chaplain's agar: 3g sodium nitrate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose, 15g agar, 1.0L distilled water, pH 6.2±0.2. Sterilize at 121°C for 15min.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 18-24h.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 6 days plate colonies are obvious
colony morphology:	small filamentous fungi have obvious colonies on Cha's agar medium. The mycelium of the plate layer is light white in the early stage, spreading and growing to cover the whole plate, producing golden yellow spores in the later stage, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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