BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 18-24h;5%CO2; |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
UACC-812(UACC-812)
BNCC338200
H22(H22)
BNCC338327
HCC-78(HCC-78)
BNCC338064
NCI-H1373(NCI-H1373)
BNCC341744
HepAD38(HepAD38)
BNCC341745
NCI-H1693(NCI-H1693)
BNCC342281