BeNa Culture Collection
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| Culture medium | BNCC370174 |
| Description | Tryptic Soy Agar with Blood and Vancomycin (TSA-V) |
| Composition | Tryptone:15.0g, Phytone:5.0g, NaCl:5.0g, Sterile defibrinated sheep blood:5%, Agar:15.0g, pH:7.3±0.2(25℃). Cool to 50℃, then add filter-sterilized vancomycin solution to a final concentration of 4µg/mL. |
| Growth conditions | 37 ℃; 24-48 hours; 5% CO2 |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white front, flat shape, smooth surface, easy to pick up, G+(blue purple), cocci |
| Sharing mode | Public welfare sharing |
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