Streptococcus parahaemolyticus|317937 |BNCC

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Streptococcus parasanguinis Whiley et al.

Culture medium	BNCC360846
Description	Modified GAM Broth
Composition	Peptone:5.0g, Tryptone:5.0g, Soy Peptone:3.0g, Yeast Extract Powder:2.5g, Beef Powder:2.2g, Digested Serum Powder:10.0g, Beef Liver Powder:1.2g, Glucose:0.5g, KH₂PO₄:2.5g, NaCl:3.0g, Soluble Starch:5.0g, L-Cysteine:0.3g, L-Arginine:1.0g, L-Tryptophan:0.2g, Sodium Thioglycolate(C₂H₃NaO₂S):0.3g, Sterile 0.1% Vitamin K1 Solution:5mL, Sterile Hemin:10mg, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium or sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. Dissolve it thoroughly and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, gray on the surface, smooth on the surface, moist texture, easy to pick up, G+(blue purple), cocci
Sharing mode	Public welfare sharing