Dark Ringed Charcoal Fungus|336334 |BNCC

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Annulohypoxylon stygium

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have distinct colonies on a comprehensive PDA culture medium, with light yellow central hyphae, white edges, and fuzzy morphology, spreading and growing towards the edge of the agar plate. There are black ring patterns on the back of the culture medium
Sharing mode	Public welfare sharing

Annulohypoxylon stygium

Storage conditions : 2~8 ℃

No. : 336334

Product format : agar slant in 14mm test tube

Validity period : 30 days

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth Conditions:25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 6 days strain layer is obvious
colony morphology	small filamentous fungi, the colony is obviously visible on the integrated PDA medium, the colony is white, the surface is yellow after maturity, and the back of the medium is dark brown with rings.
conclusion	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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