Sugarcane Laissezia|186410 |BNCC

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Laceyella sacchari

Culture medium	BNCC361877
Description	Streptomyces Medium 3
Composition	Oatmeal:20.0g, FeSO₄.7H₂O:0.001g, MnCl₂.4H₂O:0.001g, ZnSO₄.7H₂O:0.001g, Agar:18.0g, pH:7.2(25℃)
Growth conditions	55 ℃; 48-72 hours; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, flat in shape, rough and dark on the surface, dry in texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing