Bifidobacterium bifidum|186493 |BNCC

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Bifidobacterium bifidum

Culture medium	BNCC352254
Description	BBL agar medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	Prepare 1-2 of the above plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water or liquid culture medium (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L/piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing