Bifidobacterium brevis|186529 |BNCC

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Bifidobacterium breve

Culture medium	BNCC352254
Description	BBL agar medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Prepare 1-2 fresh agar plates mentioned above (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and pour it into a freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, white on the front, raised in the middle, smooth on the surface, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing