Max Kluwei yeast|338320 |BNCC

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Kluyveromyces marxianus

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	28 ℃; 24-48 hours; aerobic
Subculture procedure	(1)Prepare 1–2 agar plate medium as described above. (2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3)Add 0.5 mL sterile water to the freeze dried ampoule, mix thoroughly to reconstitute the lyophilized pellet, then pipette 200 μL onto each plate and spread evenly. (4) Incubate plates under the specified growth conditions; the strain is ready for use once growth is observed.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), cocci
Sharing mode	Public welfare sharing