BeNa Culture Collection
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| Culture medium | BNCC363915 |
| Description | NBRC Medium No. 802 |
| Composition | Polypeptone:10.0g, Yeast Powder:2.0g, MgSO4.7H2O:1.0g, Agar:15g, pH:7.0(25℃). Sterilize at 121℃ for 15 minutes. |
| Growth conditions | 37 ℃; 48-72 hours; aerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, golden yellow on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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