BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 25-28 ℃, aerobic, 5-7 days |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80℃ |
| Safety level | 1 |
| morphology | Small filamentous fungi, with obvious colonies on PDA medium, white hyphae in the early stage and light green in the later stage, dense and vigorous, spreading and growing towards the edge of the Agar plate |
| Sharing mode | Public welfare sharing |
Escherichia coli WM3064(Escherichia coli WM3064)
BNCC395800
Streptococcus sp. Grouping strain H60R(Streptococcus sp. Grouping strain H60R)
BNCC395802
Lactobacillus taiwanensis M9-1(Lactobacillus taiwanensis M9-1)
BNCC395799
Burkholderia pyrrocinia 2327(Burkholderia pyrrocinia 2327)
BNCC395783
Burkholderia cenocepacia YC40(Burkholderia cenocepacia YC40)
BNCC395737
Azotobactervinelandii(Azotobactervinelandii)
BNCC395776