Maasai bacteria|341741 |BNCC

Essential Information Certificate Related Products

Massilia neuiana

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	30 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 2-4mm Shape: Circular Edge: Neat Transparency: Opaque Color: Light Yellow Rise: Middle Rise Surface: Bright and Smooth Texture: Moist
Sharing mode	Public welfare sharing

Description

1. Name: Massilia neuensis

2. BNCC No.: 341741

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, R2A medium: yeast extract (Difco) 0.5g, prion peptone No. 3 (Difco) 0.5g, casein amino acid (Difco) 0.5g, glucose 0.5g, soluble starch 0.5g, sodium pyruvate 0.3g,K2HPO4 ;0.3g,MgSO4·7H2O 0.05g, agar powder 15.0g, disti, adjust pH to 7.2. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

4. Notes:
1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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