Clostridium|187991 |BNCC

Essential Information Related Products

Clostridium sp.

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic
Subculture procedure	① Prepare 2 of the above plates (placed in an anaerobic environment for 24 hours in advance); ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take about 0.5mL of sterile water (placed in an anaerobic environment for 24 hours in advance) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation.
Storage conditions	2-8 ℃
Safety level	1
morphology	菌落直径为1-2mm，圆形，边缘不规则，不透明，正面灰白色，中间凸起，表面光滑，质地湿润，易挑起，G＋（蓝紫色），杆菌
Sharing mode	Public welfare sharing