Enterobacter faecalis|339497 |BNCC

Essential Information Related Products

Enterococcus casseliflavus Collins et al.

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once colonies appear.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, grayish-white upper surface; flat morphology; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); cocci; Purity: Pure
Sharing mode	Public welfare sharing