Streptomyces genus|340219 |BNCC

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Streptomyces sp.

Culture medium	BNCC352255
Description	Streptomyces medium 2
Composition	Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃)
Growth conditions	28°C; 48-72h; aerobic
Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 2-4 mm; irregular shape with jagged edges; opaque, pale color; raised center; rough, wrinkled surface; easily liftable; non-pigmented; Gram-positive (blue-violet); bacillus; Purity: Pure
Sharing mode	Public welfare sharing