Trichoderma harzianum|341630 |BNCC

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Trichoderma harzianum

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28°C; 3-5 days; aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it apart with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the powder thoroughly, then dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Mycelium is villous, producing yellow spores; culture medium appears pale yellow on the reverse side.
Sharing mode	Public welfare sharing