Lactobacillus casei|186171 |BNCC

Essential Information References Related Products

Enterococcus casseliflavus

Culture medium	BNCC360797
Description	Brain-heart infusion blood agar
Composition	Brain Infusion Powder:5.0g, Heart Infusion Powder:12.5g, Peptone:5.0g, Tryptone:10.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:15.0g, pH:7.4±0.2(25℃)
Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5mL sterile water into freeze-dried tube. After complete dissolution of bacterial powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque; pale yellow upper surface; central elevation; smooth texture; moist consistency; easily liftable; G+ (blue-violet); cocci; Purity: Pure
Sharing mode	Public welfare sharing