Acetobacter Pasteurella|362975 |BNCC

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Acetobacter pasteurianus

Culture medium	BNCC363178
Description	Acetic Acid Bacteria Medium-II
Composition	20% Bean Sprout Juice:200mL, Glucose:10.0g, CaCO₃:20.0g, Ethanol:20mL, Agar:20.0g, pH:6.3(25℃)
Growth conditions	30 ℃; 48-72h; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, gray on the surface, easy to pick up, G - (red), rod-shaped
Sharing mode	Public welfare sharing