Acetobacter rhamnosus|356220 |BNCC

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Gluconacetobacter hansenii (Gossele et al.) Yamada et al.

Culture medium	BNCC372020
Description	Acetobacter xylinum Medium
Composition	Glucose:20.0g, Peptone:5.0g, Yeast extract:5.0g, Na₂HPO₄:2.7g, Citric acid(C₆H₈O₇):1.5g, Agar:18.0g
Growth conditions	26 ℃; 48-72 hours; aerobic
Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front and yellow on the back, protruding in the middle, bright on the surface, moist in texture, does not produce pigments, G - (red), rod-shaped, purity: pure
Sharing mode	Public welfare sharing