BeNa Culture Collection
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| Culture medium | BNCC372020 |
| Description | Acetobacter xylinum Medium |
| Composition | Glucose:20.0g, Peptone:5.0g, Yeast extract:5.0g, Na2HPO4:2.7g, Citric acid(C6H8O7):1.5g, Agar:18.0g |
| Growth conditions | 26 ℃; 48-72 hours; aerobic |
| Subculture procedure | Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front and yellow on the back, protruding in the middle, bright on the surface, moist in texture, does not produce pigments, G - (red), rod-shaped, purity: pure |
| Sharing mode | Public welfare sharing |
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