BeNa Culture Collection
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| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Growth conditions | 28 ℃; 7-10 days; aerobic; |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungi have obvious colonies on PDA medium, with white hyphae in the early stage and light purple in the later stage, dense and vigorous in morphology, and brown on the back of the medium |
| Sharing mode | Public welfare sharing |
Paecilomyces marquandii
Storage conditions : 2~8 ℃
No. : 356399
Product format :freeze dried, 200ul
Validity : 6 years
Biosafety level : 1, handle in ultra-clean table or safety cabinet
Growth conditions: 28 ℃, aerobic, integrated PDA,3-5 days.
Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| Item | test results |
| viability : | good viability， in 5days strain layer is obvious |
| colony morphology: |
small filamentous fungi with obvious colonies on comprehensive PDA medium, The hyphae are white at the beginning and light purple at the later stage, with dense and vigorous morphology and brown on the back of the culture medium. |
| Conclusion: | good viability, no abnormal colony morphology, qualified ; |
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