Enterococcus faecalis|189926 |BNCC

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Enterococcus faecalis

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	37°C; 24-48h; aerobic
Subculture procedure	① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve powder, then inoculate two plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular with irregular margins; opaque; grayish-white upper surface; central elevation; glossy, smooth surface; moist, viscous texture; Gram-positive (blue-violet); cocci; purity: pure
Sharing mode	Public welfare sharing