BeNa Culture Collection
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| Culture medium | BNCC352258 |
| Description | Malassezia Agar |
| Composition | Malt Extract Powder:40.0g, Ox Bile Powder:20.0g, Tween 40:10.0g, Glycerol Monoleate:2.5g, Agar:15g, pH:7.0±0.2(25℃) |
| Growth conditions | 30 ℃; 2-3 weeks; aerobic; |
| Subculture procedure | ① Dissolve the Frozen vial in a water bath (30 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation. (During the cultivation process, the agar plate should be kept moist. After the agar plate is dried, it can be opened in a safe cabinet and an appropriate amount of sterile water should be poured in.) |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 2-4mm, round, with irregular edges, opaque, yellow on the front, light color in the middle, raised in the middle, rough on the surface, wrinkled on the surface, hard in texture, dry in texture, G+(blue purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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