BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC362337 |
| Description | Chocolate Agar Plate |
| Composition | Polypeptone:18.0g, Starch:1.0g, NaCl:5.0g, Agar:15.0g, Defibrinated Sheep Blood:40mL, pH:7.4±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48h；5%CO2； |
| Subculture procedure | ① Dissolve the Frozen vial in a water bath (37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm, circular, with neat margins, opaque, grayish-white on top, convex in the center, smooth surface, glossy appearance, moist texture, easily lifted, G- (red), bacillus, purity: pure. |
| Sharing mode | Public welfare sharing |
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