BeNa Culture Collection
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| Culture medium | BNCC363070 |
| Description | Tryptic Soy Agar (TSA) |
| Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25℃) |
| Growth conditions | 30 ℃; 18-24h; aerobic; |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front and yellow on the back, protruding in the middle, smooth on the surface, moist texture, easy to pick up, does not produce pigments, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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