Klebsiella pneumoniae subsp. pneumoniae|361320 |BNCC

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Klebsiella pneumoniae subsp. pneumoniae

Culture medium	BNCC363070
Description	Tryptic Soy Agar (TSA)
Composition	Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, the edges are neat, opaque, the front is yellow, the color is light, the middle is raised, the surface is bright, the surface is smooth, the texture is moist, easy to pick up, G - (red), Bacillus
Sharing mode	Public welfare sharing