BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC362337 |
| Description | Chocolate Agar Plate |
| Composition | Polypeptone:18.0g, Starch:1.0g, NaCl:5.0g, Agar:15.0g, Defibrinated Sheep Blood:40mL, pH:7.4±0.2(25℃) |
| Growth conditions | 37 ℃; 18-24h; 5% CO2 |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube to dissolve and mix thoroughly; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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