Botrytis cinerea|364329 |BNCC

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Mycobacterium botniense

Culture medium	BNCC381233
Description	Middlebrook 7H11 Agar
Composition	KH₂PO₄:1.6g, Na₂HPO₄:1.4g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.05g, Pancreatic Digest of Casein:1.0g, ZnSO₄:0.001g, CuSO₄:0.001g, C₅H₈NNaO₄:0.5g, C₆H₈FeNO₇:0.04g, C₈H₁₁NO₃.HCl:0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, Agar:15g, pH:6.6±0.2, OADC Supplement:10%(added at 50℃)
Growth conditions	45 ℃; 2-3 weeks; aerobic;
Subculture procedure	① Dissolve the frozen tube in a water bath and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	Colony diameter of 0.5-1mm, opaque, golden yellow on the front, raised in the middle, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing