Lactobacillus lactis|364687 |BNCC

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Ruthenibacterium lactatiformans

Culture medium	BNCC363892
Description	Modified PYG Broth
Composition	Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K₂HPO₄:2.0g, Tween 80:1.0ml, CaCl₂.2H₂O:0.01g, MgSO₄.7H₂O:0.02g, K₂HPO₄:0.04g, KH₂PO₄:0.04g, NaHCO₃:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C₃H₇NO₂S.HCl:0.5g, pH:7.2±0.2(25℃)
Growth conditions	37 ℃; 3-5 days; anaerobic;
Subculture procedure	① Dissolve the Frozen vial tube in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing