BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360798 |
| Description | Colombia Agar Chocolate Plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48h；5%CO2； |
| Subculture procedure | Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Disinfect the surface of the carrier tablet and open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, dark yellow on the front, raised in the middle, smooth surface, moist texture, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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