Porcine red spot erysipelas|368407 |BNCC

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Erysipelothrix rhusiopathiae

Culture medium	BNCC360798
Description	Colombia Agar Chocolate Plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48h；5%CO2；
Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Disinfect the surface of the carrier tablet and open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, dark yellow on the front, raised in the middle, smooth surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing