Shortwave Pseudomonas aeruginosa|195924 |BNCC

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Brevundimonas diminuta

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	菌落直径为1-2mm，圆形，边缘整齐，不透明，正面黄色，颜色较浅，中间凸起，表面明亮，表面光滑，质地湿润，易挑起，G－（红），杆菌
Sharing mode	Public welfare sharing