Baker's yeast|186748 |BNCC

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Saccharomyces cerevisiae

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	30°C; 24-48h; aerobic
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to break it; shatter with forceps; ③ Pipette 0.5 mL liquid medium into a freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2mm, circular, with neat edges; opaque, white on top, centrally raised, smooth surface, easily liftable; G+ (blue-violet), cocci
Sharing mode	Public welfare sharing