BeNa Culture Collection
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| Culture medium | BNCC380909 |
| Description | YpSs Agar |
| Composition | Yeast Extract:4g, Soluble Starch:15g, K2HPO4:1g, MgSO4.7H2O:0.5g, Agar:18g, Water:1L, pH:7.2±0.2(25℃) |
| Growth conditions | 24 ℃; 7-15 days; aerobic; |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, yellow on the front, raised in the middle, rough on the surface, dry texture, G+(blue purple), Bacillus |
| Sharing mode | Public welfare sharing |
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