BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic |
| Subculture procedure | ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate is about 200 μ L). Place the plates in an anaerobic environment for 24 hours to equilibrate. ④ Apply evenly and transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque, grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; Gram-positive (blue-violet); cocci; purity: pure |
| Sharing mode | Public welfare sharing |
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