BeNa Culture Collection
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| Culture medium | BNCC352256 |
| Description | Czapek Dox Agar |
| Composition | NaNO3:3.0g, K2HPO4:1.0g, MgSO4:0.5g, KCl:0.5g, FeSO4:0.01g, Sucrose:30.0g, Agar:15.0g |
| Growth conditions | 24 ℃; 7-10 days; aerobic; |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Spread and grow, with white mycelium on the front and yellow brown on the back, with radial grooves. Spore production is evident when cultured on CPDA |
| Sharing mode | Public welfare sharing |
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