BeNa Culture Collection
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| Culture medium | BNCC362653 |
| Description | GYM Streptomyces Agar |
| Composition | Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO3:2.0g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 28 ℃; 2-4 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Colony diameter: 2-4 mm; circular with irregular margins; opaque; light brown upper surface; convex center; glossy surface; wrinkled texture; firm and dry consistency; Gram-positive (blue-violet); bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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