Vibrio Hung's Butyrate|388765 |BNCC

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Butyrivibrio hungatei

Culture medium	BNCC391263
Description	Modified PYG Broth with Rumen Fluid (30ml/L)
Composition	Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K₂HPO₄:2.0g, Tween 80:1.0ml, CaCl₂.2H₂O:0.01g, MgSO₄.7H₂O:0.02g, K₂HPO₄:0.04g, KH₂PO₄:0.04g, NaHCO₃:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C₃H₇NO₂S.HCl:0.5mg
Growth conditions	37 ℃; 3-5 days; anaerobic;
Subculture procedure	① Dissolve the Frozen vial tube in a water bath (bacteria 37 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into a test tube (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing