BeNa Culture Collection
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| Culture medium | BNCC360815 |
| Description | Nitrogen fixing medium |
| Composition | KH2PO4:0.2g, K2HPO4:0.8g, MgSO4.7H2O:0.2g, CaSO4.2H2O:0.1g, FeCl3:Trace, Na2MoO4.2H2O:Trace, Yeast Extract:0.5g, Mannitol:20.0g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 30 ℃; 3-7 days; aerobic; |
| Subculture procedure | ① Dissolve the Frozen vial in a water bath (30 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, pink on the front, raised in the middle, smooth on the surface, moist texture, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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