BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC372404 |
| Description | Reinforced Clostridial Medium (RCM) |
| Composition | Yeast Extract:3.0g, Beef Infusion Powder:10.0g, Glucose:5.0g, Pancreatic Digest of Casein:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, CH3COONa:3.0g, Agar:0.5g, pH:6.8±0.1(25℃) |
| Growth conditions | 37 ℃; 18-24h; anaerobic |
| Subculture procedure | ① Prepare one 18mm test tube of liquid culture medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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