Rhizopus creeping|373215 |BNCC

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Rhizopus stolonifer

Culture medium	BNCC352233
Description	Potato glucose agar
Composition	Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃)
Growth conditions	28 ℃; 3-5 days; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Spreading growth, produces black spores abundantly, irregular margin, opaque, grayish-white on obverse side, flat form, rough and dull surface, dry texture, purity: pure culture, on PDA medium with vigorous hyphal growth at 24 hours and production of black spores after 48 hours.
Sharing mode	Public welfare sharing