BeNa Culture Collection
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| Culture medium | BNCC368149 |
| Description | L-Histidine Medium |
| Composition | L-Histidine:4.043g |
| Growth conditions | 37 ℃; 18-24h; aerobic; |
| Subculture procedure | Draw a line on a flat Agar plate and prepare one 50mL nutrient broth triangular bottle with typical bacterial colonies; ② Use sterile tweezers to remove one from the biosafety cabinet and place it in a triangular bottle; ③ Place the triangular flask on a shaking table at 37 ℃ for shaking cultivation, and achieve a viable bacterial count of (1-2) x10 ^ 9/mL. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | Verification method: Inoculate the strain into nutrient broth medium and culture on a shaker at 37 ℃ for 18-24 hours. The bacterial solution is significantly turbid. Then dip the inoculation ring into the bacterial solution and streak culture on the medium containing L-histidine and the medium without L-histidine, respectively. After 24 hours of cultivation at 37 ℃, observe. The strain grows well on the surface of the L-histidine containing medium Agar plate (left image), but is inhibited in the absence of L-histidine medium Agar plate, and does not grow or grows a few colonies (right image) |
| Sharing mode | Public welfare sharing |
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