Histidine deficient Salmonella typhimurium TA1537|381570 |BNCC

BeNa Culture Collection

Histidine deficient Salmonella typhimurium TA1537-BNCC
  • BNCC
  • Salmonella typhimurium(his) TA1537-BNCC

Salmonella typhimurium(his) TA1537

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  • Price: $ 409
  • number:BNCC381570
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    Verification method: Inoculate the strain into nutrient broth medium and culture on a shaker at 37 ℃ for 18-24 hours. The bacterial solution is significantly turbid. Then dip the inoculation ring into the bacterial solution and streak culture on the medium containing L-histidine and the medium without L-histidine, respectively. After 24 hours of cultivation at 37 ℃, observe. The strain grows well on the surface of the L-histidine containing medium Agar plate (left image), but is inhibited in the absence of L-histidine medium Agar plate, and does not grow or grows a few colonies (right image)
Essential Information References Related Products
Salmonella typhimurium(his) TA1537
Culture medium BNCC368149
Description L-Histidine Medium
Composition L-Histidine:4.043g
Growth conditions 37 ℃; 18-24h; aerobic;
Subculture procedure Draw a line on a flat Agar plate and prepare one 50mL nutrient broth triangular bottle with typical bacterial colonies; ② Use sterile tweezers to remove one from the biosafety cabinet and place it in a triangular bottle; ③ Place the triangular flask on a shaking table at 37 ℃ for shaking cultivation, and achieve a viable bacterial count of (1-2) x10 ^ 9/mL.
Storage conditions 2-8 ℃
Safety level 2
morphology Verification method: Inoculate the strain into nutrient broth medium and culture on a shaker at 37 ℃ for 18-24 hours. The bacterial solution is significantly turbid. Then dip the inoculation ring into the bacterial solution and streak culture on the medium containing L-histidine and the medium without L-histidine, respectively. After 24 hours of cultivation at 37 ℃, observe. The strain grows well on the surface of the L-histidine containing medium Agar plate (left image), but is inhibited in the absence of L-histidine medium Agar plate, and does not grow or grows a few colonies (right image)
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