BeNa Culture Collection
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| Culture medium | BNCC380934 |
| Description | Malt Extract Agar with 20% Sucrose |
| Composition | Malt Extract:40g, Sucrose:200g, Water:960mL, Agar:18g, pH:7.0±0.2. Sterilize at 121℃ for 15 minutes. |
| Growth conditions | 25 ℃; 7-15 days; aerobic; |
| Subculture procedure | Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, brown, protruding in the middle, rough surface, dry texture, G+(blue purple), square shaped |
| Sharing mode | Public welfare sharing |
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