Neisseria gonorrhoeae BDMS T4145|247784 |BNCC

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Neisseria gonorrhoeae (Zopf) Trevisan

Culture medium	BNCC360798
Description	Colombia Agar Chocolate Plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; 5% CO2+95% air
Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist in texture, does not produce pigments, G - (red), cocci, purity: pure
Sharing mode	Public welfare sharing