New type of cryptosphere yeast|254020 |BNCC

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Cryptococcus neoformans (Sanfelice) Vuillemin

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	28 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for repeated cultivation. The colony diameter is 1-2mm, round, with neat edges, opaque, milky white on the front, raised in the middle, smooth on the surface, sticky and moist in texture. G+(blue purple), cocci, purity: pure
Storage conditions	2-8 ℃
Safety level	2
morphology	菌落直径为1-2mm，圆形，边缘整齐，不透明，正面乳白色，中间凸起，表面光滑，质地黏稠，质地湿润，G＋（蓝紫色），球菌，纯度：纯
Sharing mode	Public welfare sharing